27 cities
| City | Rank | 2025 Pop. ↓ | 2020 Pop. | Change | Density | Area (mi²) | Type |
|---|---|---|---|---|---|---|---|
| Charleston township | 1 | 3,471 | 3,557 | -0.46% | 67 | 52.16 | Township |
| Wellsboro | 2 | 3,399 | 3,469 | -0.41% | 693 | 4.90 | Borough |
| Mansfield | 3 | 3,200 | 2,845 | 2.27% | 1,699 | 1.88 | Borough |
| Delmar township | 4 | 2,800 | 2,795 | 0.29% | 34 | 82.23 | Township |
| Morris township | 5 | 2,697 | 2,771 | -0.63% | 137 | 19.72 | Township |
| Covington township | 6 | 2,263 | 2,204 | 0.53% | 96 | 23.70 | Township |
| Liberty | 7 | 2,209 | 2,351 | -1.25% | 1,545 | 1.43 | Borough |
| Richmond township | 8 | 2,117 | 2,162 | -0.47% | 42 | 50.24 | Township |
| Liberty township | 9 | 1,807 | 1,797 | -0.17% | 67 | 26.96 | Township |
| Elkland | 10 | 1,767 | 1,826 | -0.68% | 676 | 2.61 | Borough |
| Liberty township | 11 | 1,745 | 1,807 | -0.97% | 74 | 23.58 | Township |
| Liberty township | 12 | 1,553 | 1,607 | -0.26% | 18 | 84.55 | Township |
| Blossburg | 13 | 1,490 | 1,537 | -0.6% | 321 | 4.65 | Borough |
| Liberty township | 14 | 1,471 | 1,394 | 1.73% | 100 | 14.76 | Township |
| Sullivan township | 15 | 1,439 | 1,471 | -0.41% | 34 | 42.59 | Township |
| Liberty township | 16 | 1,411 | 1,366 | 0.79% | 87 | 16.22 | Township |
| Liberty township | 17 | 1,389 | 1,383 | 0.29% | 53 | 26.19 | Township |
| Middlebury township | 18 | 1,292 | 1,306 | -0.15% | 26 | 49.81 | Township |
| Liberty township | 19 | 1,125 | 1,129 | 0.18% | 38 | 29.55 | Township |
| Westfield | 20 | 1,087 | 1,111 | -0.46% | 975 | 1.11 | Borough |
| Liberty township | 21 | 1,019 | 1,037 | -0.49% | 16 | 63.28 | Township |
| Covington township | 22 | 1,008 | 1,030 | -0.49% | 28 | 36.40 | Township |
| Lawrenceville | 23 | 685 | 692 | -0.15% | 1,114 | 0.61 | Borough |
| Knoxville | 24 | 664 | 681 | -0.45% | 1,071 | 0.62 | Borough |
| Tioga | 25 | 595 | 612 | -0.5% | 1,282 | 0.46 | Borough |
| Liberty | 26 | 228 | 232 | -0.44% | 479 | 0.48 | Borough |
| Roseville | 27 | 175 | 177 | 0% | 368 | 0.47 | Borough |
Charleston township is the largest city in Tioga County, with a population of 3,471.
Roseville is the smallest city in Tioga County.
There are 27 cities in Tioga County, Pennsylvania.