28 cities
| City | Rank | 2025 Pop. ↓ | 2020 Pop. | Annual Change | Density | Area (mi²) | Type |
|---|---|---|---|---|---|---|---|
| Cumberland | 1 | 6,006 | 6,375 | -1.35% | 157 | 38 | Township |
| Jefferson | 2 | 5,041 | 5,209 | -0.45% | 220 | 23 | Township |
| Jefferson | 3 | 3,916 | 3,757 | 0.67% | 117 | 34 | Township |
| Waynesburg | 4 | 3,628 | 3,992 | -1.97% | 4,507 | 1 | Borough |
| Jefferson | 5 | 2,439 | 2,389 | 0.66% | 153 | 16 | Township |
| Jefferson | 6 | 2,189 | 2,343 | -1.4% | 102 | 22 | Township |
| Dunkard | 7 | 2,015 | 2,168 | -1.66% | 64 | 32 | Township |
| Jefferson | 8 | 1,925 | 2,008 | -0.62% | 96 | 20 | Township |
| Jefferson | 9 | 1,844 | 1,919 | -0.65% | 75 | 25 | Township |
| Aleppo | 10 | 1,774 | 1,825 | -0.17% | 997 | 2 | Township |
| Jefferson | 11 | 1,393 | 1,431 | -0.5% | 34 | 41 | Township |
| Perry | 12 | 1,311 | 1,394 | -1.58% | 43 | 30 | Township |
| Jefferson | 13 | 1,118 | 1,121 | 0% | 49 | 23 | Township |
| Center | 14 | 1,041 | 1,109 | -1.61% | 21 | 49 | Township |
| Deemston | 15 | 736 | 753 | -0.54% | 77 | 10 | Borough |
| Richhill | 16 | 730 | 775 | -0.82% | 13 | 54 | Township |
| Jefferson | 17 | 655 | 652 | 0% | 1,081 | 1 | Borough |
| Whiteley | 18 | 544 | 569 | -0.37% | 17 | 32 | Township |
| Aleppo | 19 | 396 | 422 | -1.74% | 14 | 28 | Township |
| Carmichaels | 20 | 395 | 425 | -1.5% | 2,394 | 0 | Borough |
| Rices Landing | 21 | 389 | 419 | -1.52% | 490 | 1 | Borough |
| Greene | 22 | 383 | 397 | -1.29% | 21 | 19 | Township |
| Jefferson | 23 | 361 | 360 | 0.28% | 15 | 24 | Township |
| Springhill | 24 | 294 | 319 | -1.67% | 13 | 22 | Township |
| Greensboro | 25 | 247 | 266 | -1.59% | 2,287 | 0 | Borough |
| Jefferson | 26 | 234 | 252 | -1.68% | 1,245 | 0 | Borough |
| Gilmore | 27 | 221 | 240 | -1.78% | 10 | 23 | Township |
| Clarksville | 28 | 182 | 194 | -1.62% | 1,876 | 0 | Borough |
Cumberland is the largest city in Greene County, with a population of 6,006.
Clarksville is the smallest city in Greene County, with a population of 182.
There are 28 cities in Greene County, Pennsylvania.