28 cities
| City | Rank | 2026 Pop. ↓ | 2020 Pop. | Annual Change | Density | Area (mi²) | Type |
|---|---|---|---|---|---|---|---|
| Cumberland | 1 | 5,924 | 6,375 | -1.37% | 155 | 38 | Township |
| Jefferson | 2 | 5,018 | 5,209 | -0.46% | 219 | 23 | Township |
| Jefferson | 3 | 3,942 | 3,757 | 0.66% | 117 | 34 | Township |
| Waynesburg | 4 | 3,555 | 3,992 | -2.01% | 4,416 | 1 | Borough |
| Jefferson | 5 | 2,455 | 2,389 | 0.66% | 154 | 16 | Township |
| Jefferson | 6 | 2,158 | 2,343 | -1.42% | 100 | 22 | Township |
| Dunkard | 7 | 1,981 | 2,168 | -1.69% | 63 | 32 | Township |
| Jefferson | 8 | 1,913 | 2,008 | -0.62% | 96 | 20 | Township |
| Jefferson | 9 | 1,832 | 1,919 | -0.65% | 74 | 25 | Township |
| Aleppo | 10 | 1,771 | 1,825 | -0.17% | 995 | 2 | Township |
| Jefferson | 11 | 1,386 | 1,431 | -0.5% | 34 | 41 | Township |
| Perry | 12 | 1,290 | 1,394 | -1.6% | 43 | 30 | Township |
| Jefferson | 13 | 1,118 | 1,121 | 0% | 49 | 23 | Township |
| Center | 14 | 1,024 | 1,109 | -1.63% | 21 | 49 | Township |
| Deemston | 15 | 733 | 753 | -0.41% | 76 | 10 | Borough |
| Richhill | 16 | 724 | 775 | -0.82% | 13 | 54 | Township |
| Jefferson | 17 | 656 | 652 | 0.15% | 1,083 | 1 | Borough |
| Whiteley | 18 | 542 | 569 | -0.37% | 17 | 32 | Township |
| Aleppo | 19 | 389 | 422 | -1.77% | 14 | 28 | Township |
| Carmichaels | 20 | 389 | 425 | -1.52% | 2,358 | 0 | Borough |
| Rices Landing | 21 | 383 | 419 | -1.54% | 482 | 1 | Borough |
| Greene | 22 | 378 | 397 | -1.31% | 20 | 19 | Township |
| Jefferson | 23 | 362 | 360 | 0.28% | 15 | 24 | Township |
| Springhill | 24 | 289 | 319 | -1.7% | 13 | 22 | Township |
| Greensboro | 25 | 243 | 266 | -1.62% | 2,250 | 0 | Borough |
| Jefferson | 26 | 231 | 252 | -1.28% | 1,229 | 0 | Borough |
| Gilmore | 27 | 217 | 240 | -1.81% | 9 | 23 | Township |
| Clarksville | 28 | 180 | 194 | -1.1% | 1,856 | 0 | Borough |
Cumberland is the largest city in Greene County, with a population of 5,924.
Clarksville is the smallest city in Greene County, with a population of 180.
There are 28 cities in Greene County, Pennsylvania.