7 cities
| City | Rank | 2026 Pop. ↓ | 2020 Pop. | Annual Change | Density | Area (mi²) | Type |
|---|---|---|---|---|---|---|---|
| Schertz | 1 | 45,566 | 42,152 | 1.26% | 1,202 | 38 | City |
| Seguin | 2 | 43,443 | 29,480 | 5.66% | 1,085 | 40 | City |
| Cibolo | 3 | 40,317 | 32,813 | 3.2% | 1,912 | 21 | City |
| Marion | 4 | 1,122 | 1,050 | 1.08% | 1,437 | 1 | City |
| New Berlin | 5 | 766 | 601 | 3.79% | 80 | 10 | City |
| Staples | 6 | 188 | 193 | -0.53% | 126 | 1 | City |
| Kingsbury | 7 | 144 | 140 | 0.7% | 56 | 3 | City |
Schertz is the largest city in Guadalupe County, with a population of 45,566.
Kingsbury is the smallest city in Guadalupe County, with a population of 144.
There are 7 cities in Guadalupe County, Texas.