9 cities
| City | Rank | 2025 Pop. ↓ | 2020 Pop. | Change | Density | Area (mi²) | Type |
|---|---|---|---|---|---|---|---|
| Schertz | 1 | 43,970 | 42,142 | 0.84% | 1,327 | 33.13 | City |
| Seguin | 2 | 40,375 | 29,470 | 5.71% | 1,009 | 40.03 | City |
| Cibolo | 3 | 38,736 | 32,830 | 3.14% | 1,845 | 21 | City |
| McQueeney | 4 | 2,328 | 2,907 | -0.6% | 569 | 4.09 | CDP |
| Marion | 5 | 1,094 | 1,044 | 0.92% | 1,401 | 0.78 | City |
| Geronimo | 6 | 1,086 | 793 | 0.37% | 136 | 7.99 | CDP |
| New Berlin | 7 | 803 | 662 | 3.61% | 83 | 9.63 | City |
| Staples | 8 | 182 | 189 | -0.55% | 122 | 1.50 | City |
| Kingsbury | 9 | 137 | 136 | 0% | 53 | 2.58 | City |
Schertz is the largest city in Guadalupe County, with a population of 43,970.
Kingsbury is the smallest city in Guadalupe County.
There are 9 cities in Guadalupe County, Texas.