7 cities
| City | Rank | 2025 Pop. ↓ | 2020 Pop. | Annual Change | Density | Area (mi²) | Type |
|---|---|---|---|---|---|---|---|
| Schertz | 1 | 44,997 | 42,152 | 1.28% | 1,187 | 38 | City |
| Seguin | 2 | 41,116 | 29,480 | 6% | 1,027 | 40 | City |
| Cibolo | 3 | 39,066 | 32,813 | 3.31% | 1,853 | 21 | City |
| Marion | 4 | 1,110 | 1,050 | 1.09% | 1,421 | 1 | City |
| New Berlin | 5 | 738 | 601 | 3.8% | 77 | 10 | City |
| Staples | 6 | 189 | 193 | -0.53% | 126 | 1 | City |
| Kingsbury | 7 | 143 | 140 | 0% | 56 | 3 | City |
Schertz is the largest city in Guadalupe County, with a population of 44,997.
Kingsbury is the smallest city in Guadalupe County, with a population of 143.
There are 7 cities in Guadalupe County, Texas.