8 cities
| City | Rank | 2025 Pop. ↓ | 2020 Pop. | Change | Density | Area (mi²) | Type |
|---|---|---|---|---|---|---|---|
| Hillsboro | 1 | 6,702 | 6,489 | 0.65% | 1,224 | 5.47 | City |
| Greenfield | 2 | 4,332 | 4,341 | -0.05% | 2,077 | 2.09 | Village |
| Lynchburg | 3 | 1,545 | 1,515 | 0.39% | 1,674 | 0.92 | Village |
| Leesburg | 4 | 1,288 | 1,268 | 0.31% | 1,103 | 1.17 | Village |
| Buford | 5 | 461 | 280 | 1.54% | 311 | 1.48 | CDP |
| Mowrystown | 6 | 385 | 387 | 0% | 799 | 0.48 | Village |
| Highland | 7 | 227 | 229 | 0% | 1,335 | 0.17 | Village |
| Sinking Spring | 8 | 121 | 121 | 0% | 259 | 0.47 | Village |
Hillsboro is the largest city in Highland County, with a population of 6,702.
Sinking Spring is the smallest city in Highland County.
There are 8 cities in Highland County, Ohio.