9 cities
| City | Rank | 2025 Pop. ↓ | 2020 Pop. | Annual Change | Density | Area (mi²) | Type |
|---|---|---|---|---|---|---|---|
| Greenville | 1 | 26,392 | 29,504 | -2.31% | 981 | 27 | City |
| Leland | 2 | 3,542 | 3,964 | -2.34% | 989 | 4 | City |
| Hollandale | 3 | 2,074 | 2,317 | -2.31% | 936 | 2 | City |
| Metcalfe | 4 | 729 | 813 | -2.28% | 727 | 1 | Town |
| Elizabeth | 5 | 324 | 275 | 7.28% | 1,022 | 0 | CDP |
| Glen Allan | 6 | 292 | 148 | 6.96% | 64 | 5 | CDP |
| Arcola | 7 | 276 | 304 | -2.13% | 1,260 | 0 | Town |
| Winterville | 8 | 131 | 52 | 7.38% | 51 | 3 | CDP |
| Stoneville | 9 | 30 | 44 | 7.14% | 16 | 2 | CDP |
Greenville is the largest city in Washington County, with a population of 26,392.
Stoneville is the smallest city in Washington County, with a population of 30.
There are 9 cities in Washington County, Mississippi.