4 cities
| City | Rank | 2025 Pop. ↓ | 2020 Pop. | Change | Density | Area (mi²) | Type |
|---|---|---|---|---|---|---|---|
| Mayersville | 1 | 391 | 431 | -2% | 355 | 1.10 | Town |
| Grace | 2 | 284 | 130 | 7.17% | 59 | 4.80 | CDP |
| Eagle Bend | 3 | 253 | 504 | 7.2% | 151 | 1.67 | CDP |
| Valley Park | 4 | 150 | 258 | 7.14% | 33 | 4.54 | CDP |
Mayersville is the largest city in Issaquena County, with a population of 391.
Valley Park is the smallest city in Issaquena County.
There are 4 cities in Issaquena County, Mississippi.