6 cities
| City | Rank | 2025 Pop. ↓ | 2020 Pop. | Change | Density | Area (mi²) | Type |
|---|---|---|---|---|---|---|---|
| Fredericktown | 1 | 4,574 | 4,433 | 0.62% | 1,058 | 4.33 | City |
| Junction City | 2 | 281 | 283 | 0% | 785 | 0.36 | Village |
| Cobalt | 3 | 262 | 262 | 0% | 1,758 | 0.15 | Village |
| Mine La Motte | 4 | 208 | 387 | -4.15% | 64 | 3.27 | CDP |
| Marquand | 5 | 188 | 188 | 0% | 790 | 0.24 | City |
| Cherokee Pass | 6 | 186 | 261 | -2.1% | 89 | 2.08 | CDP |
Fredericktown is the largest city in Madison County, with a population of 4,574.
Cherokee Pass is the smallest city in Madison County.
There are 6 cities in Madison County, Missouri.